Simultaneous quantification of human cytomegalovirus (HCMV)‐specific CD4+ and CD8+ T cells by a novel method using monocyte‐derived HCMV‐infected immature dendritic cells

Immature dendritic cells (DC) infected with an endotheliotropic (Huv+) and leukotropic (Leuk+) human cytomegalovirus (HCMV) strain were used as a stimulus to determine functional HCMV‐specific CD4+ and CD8+ T cells. Infected DC were co‐cultured with autologous peripheral blood mononuclear cells and...

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Bibliographic Details
Published in:	European journal of immunology Vol. 35; no. 6; pp. 1795 - 1804
Main Authors:	Lozza, Laura, Lilleri, Daniele, Percivalle, Elena, Fornara, Chiara, Comolli, Giuditta, Revello, Maria Grazia, Gerna, Giuseppe
Format:	Journal Article
Language:	English
Published:	Weinheim WILEY‐VCH Verlag 01-06-2005
Subjects:	CD4 Lymphocyte Count CD8-Positive T-Lymphocytes - immunology Coculture Techniques Cytokine flow cytometry Cytomegalovirus - immunology Dendritic cells Dendritic Cells - physiology Dendritic Cells - virology Flow Cytometry HCMV Humans Immunocompromised Host Interferon-gamma - biosynthesis Lymphocyte Activation Lymphocyte Count Reproducibility of Results Specific T cells
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