Immunomodulatory properties of D-sorbitol/D-mannitol incorporated linear step-growth Co-polymers

• Published in: International journal of polymeric materials Vol. 72; no. 9; pp. 690 - 701
• Main Authors: Kiran, S., Parvathy, J., Sukumaran, Thenmozhi, Varghese, Jeslin, Lakshmi, S, Kumar, Sreesha S., Babu, Anu, Harikumar, Kuzhuvelil B., Ragupathy, Lakshminarayanan
• Format: Journal Article
• Language: English
• Published: Philadelphia Taylor & Francis 13-06-2023; Taylor & Francis Ltd
• Subjects: Adjuvants; Biocompatibility; Chemical synthesis; Copolymers; D-mannitol/D-sorbitol based co-polymers; immunomodulatory effects; Mannitol; Polyethylene glycol; Polymers; Sorbitol; Toxicity; toxicity studies; vaccine adjuvant
• ISSN: 0091-4037; 1563-535X
• DOI: 10.1080/00914037.2022.2052726

A range of D-sorbitol/D-mannitol incorporated co-polymers were synthesized via solvent free enzyme catalyzed polycondensation with adipic acid and polyethylene glycol. In-vitro cytotoxic studies on breast cancer and fibroblast cell lines showed that the co-polymers are biocompatible up to 500 and 250 µg/mL, respectively. However, THP-1 and RAW 264.7 cell lines showed a dose dependent cytotoxicity. Furthermore, these co-polymers did not produce excessive cytokines (TNFα/IL-6) and chemokines (CXCL-5/CXCL-10) when interacting with THP-1 cells, thereby, not causing any inflammation. Whilst, the expression of IL-1b, CXCL-1 and IFNβ were upregulated. The results suggest that these co-polymers could be promising vaccine adjuvants.