Antifungal activity of essential oil-encapsulated lipid nanoemulsions formulations against leaf spot disease on tomato caused by Alternaria alternata

• Published in: Archiv für Phytopathologie und Pflanzenschutz Vol. 55; no. 2; pp. 235 - 257
• Main Authors: Nguyen, Minh-Hiep, Tran, Thi-Ngoc-Mai, Vu, Ngoc-Bich-Dao
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 20-01-2022; Taylor & Francis Ltd
• Subjects: Alternaria alternata; Antifungal activity; Bioassays; Cinnamon; combined formulation; Encapsulation; Essential oils; Fungicides; Garlic; leaf spot disease; Leafspot; Leaves; lipid nanoemulsions; Lipids; Nanoemulsions; Oils & fats; Plant growth; Tomatoes
• ISSN: 0323-5408; 1477-2906
• DOI: 10.1080/03235408.2021.2015887

This study aimed to find out the optimal formulation of essential oil-encapsulated lipid nanoemulsions (EO-LNs) by comparing antifungal activity against leaf spot disease caused by Alternaria alternata on tomato plants of 10 different EO-LNs, followed by their binary and ternary combinations. Firstly, 10 EO-LNs and their combined formulations were prepared and their in-vitro antifungal activity was evaluated using petri dish bioassay. Next, ex-vitro disease-prevention and disease-treatment experiments of the optimal EO-LNs formulation were carried out in the greenhouse. The results indicated that there was a significant difference in in-vitro antifungal activity against Alternaria alternata among 10 EO-LNs and their combined formulations. Overall, NaChiGarCin (the ternary combination of chili oil-encapsulated LNs, garlic oil-encapsulated LNs, cinnamon oil-encapsulated LNs) exhibited the highest in-vitro antifungal activity. Under ex-vitro condition, NaChiGarCin at an EO-concentration of 200 ppm showed the highest disease-preventing and treating effectiveness (above 80%) and showed no negative effect on the plant growth.
Supplemental data for this article is available online at http://dx.doi.org/10.1080/03235408.2021.2015887 .