Uninduced adipose-derived stem cells repair the defect of full-thickness hyaline cartilage

• Published in: Chinese journal of traumatology Vol. 12; no. 2; pp. 92 - 97
• Main Author: 张海宁 李磊 冷萍 王英振 吕成昱
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01-04-2009; Department of Joint Surgery,Affiliated Hospital of Tsingtao University,Tsingtao 266003,China%Department of Orthopaedics,Donggang Hospital of Rizhao,Rizhao 276800,China%Department of Pharmacology,Affiliated Hospital of Tsingtao University,Tsingtao 266003,China
• Subjects: Adipose tissue; Adipose Tissue - cytology; Alginates; Animals; Cartilage, Articular - injuries; Cartilage, Articular - surgery; Cells, Cultured; Glucuronic Acid; Hexuronic Acids; Hyaline cartilage; Mesenchymal Stromal Cells - physiology; Rabbits; Stem cells; Tissue Engineering; Wounds and injuries; 干细胞修复; 胶原纤维细胞; 脂肪组织; 藻酸钙凝胶; 软骨缺损; 透明软骨; Adipose tissue; Hyaline cartilage; Wounds and injuries; Stem cells
• ISSN: 1008-1275
• DOI: 10.3760/cma.j.issn.1008-1275.2009.02.006

Objective： To testify the effect of the stem cells derived from the widely distributed fat tissue on repairing full-thickness hyaline cartilage defects.
Methods： Adipose-derived stem cells （ADSCs） were derived from adipose tissue and cultured in vitro. Twenty- seven New Zealand white rabbits were divided into three groups randomly. The cultured ADSCs mixed with calcium alginate gel were used to fill the full-thickness hyaline cartilage defects created at the patellafemoral joint, and the defects repaired with gel or without treatment served as control groups. After 4, 8 and 12 weeks, the reconstructed tissue was evaluated macroscopically and microscopically. Histological analysis and qualitative scoring were also performed to detect the outcome.
Results： Full thickness hyaline cartilage defects were repaired completely with ADSCs-derived tissue. The result was better in ADSCs group than the control ones. The microstructure of reconstructed tissue with ADSCs was similar to that of hyaline cartilage and contained more cells and regular matrix fibers, being better than other groups. Plenty of collagen fibers around cells could be seen under transmission electron microscopy. Statistical analysis revealed a significant difference in comparison with other groups at each time point （t=4.360, P〈0.01）.
Conclusion： These results indicate that stem cells derived from mature adipose without induction possess the ability to repair cartilage defects