Different respiratory defective phenotypes of Neurospora crassa and Saccharomyces cerevisiae after inactivation of the gene encoding the mitochondrial acyl carrier protein

• Published in: Current genetics Vol. 29; no. 1; pp. 10 - 17
• Main Authors: Schneider, R, Massow, M, Lisowsky, T, Weiss, H
• Format: Journal Article
• Language: English
• Published: United States 01-12-1995
• Subjects: acide gras; acidos grasos; Acyl Carrier Protein - genetics; Acyl Carrier Protein - isolation & purification; Base Sequence; biosintesis; biosynthese; biosynthesis; Electron Transport Complex I; Electron Transport Complex IV - genetics; Electron Transport Complex IV - metabolism; fatty acids; gene; Gene Expression Regulation, Fungal; genes; Mitochondria - chemistry; Mitochondria - genetics; Molecular Sequence Data; Mutagenesis; NADH, NADPH Oxidoreductases - genetics; Neurospora crassa; Neurospora crassa - genetics; Neurospora crassa - growth & development; oxidoreductases; oxidorreductasas; oxydoreductase; Phenotype; Phospholipids - biosynthesis; Phospholipids - chemistry; proteinas; proteine; proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development
• ISSN: 0172-8083; 1432-0983
• DOI: 10.1007/BF00313188

The nuclear genes (acp-1, ACP1) encoding the mitochondrial acyl carrier protein were disrupted in Neurospora crassa and Saccharomyces cerevisiae. In N. crassa acp-1 is a peripheral subunit of the respiratory NADH: ubiquinone oxidoreductase (complex 1). S. cerevisiae lacks complex 1 and its ACP1 appears to be located in the mitochondrial matrix. The loss of acp-1 in N. crassa causes two biochemical lesions. Firstly, the peripheral part of complex 1 is not assembled, and the membrane part is not properly assembled. The respiratory ubiquinol:cytochrome c oxidoreductase (complex 3) and cytochrome c oxidase (complex 4) are made in normal amounts. Secondly, the lysophospholipid content of mitochondrial membranes is increased four-fold. In S. cerevisiae, the loss of ACP1 leads to a pleiotropic respiratory deficient phenotype.