Characterization of a Gram-Positive Bacterium from the Proventriculus of Budgerigars (Melopsittacus undulatus)

The cellular, cultural, and biochemical characteristics of eight isolates of a large gram-positive bacillus that are commonly observed as apparently normal flora in the proventriculus of budgerigars (Melopsittacus undulatus) were determined. The bacterium was highly pleomorphic and changed markedly...

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Published in:Avian diseases Vol. 34; no. 3; pp. 779 - 786
Main Authors: Scanlan, Charles M., Graham, David L.
Format: Journal Article
Language:English
Published: United States American Association of Avian Pathologists, Inc 01-07-1990
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Summary:The cellular, cultural, and biochemical characteristics of eight isolates of a large gram-positive bacillus that are commonly observed as apparently normal flora in the proventriculus of budgerigars (Melopsittacus undulatus) were determined. The bacterium was highly pleomorphic and changed markedly in both diameter and length when subcultured on agar media. The bacterium was facultative anaerobic and capnophilic, hemolytic on blood agar, and formed flat colonies with irregular edges after incubation for several days. All isolates grew on sodium azide agar but did not grow on MacConkey agar. The isolates were catalase-negative and oxidase-negative and did not reduce nitrate. All isolates failed to utilize arginine, lysine, ornithine or tryptophane but produced acid from glucose, galactose, levulose, maltose, melibiose, starch, and sucrose. All isolates produced acetoin from glucose and hydrolyzed esculin. The eight isolates could not be identified to either genus or species level based on the descriptions of currently classified organisms in the division Firmicutes as described in Bergey's Manual of Systematic Bacteriology. /// Se determinaron las características celulares, culturales y bioquímicas de ocho cepas de bacilos gram positivos grandes que comúmente son observados como pertenecientes a la flora normal del proventrículo de los periquitos (Melopsittacus undulatus). La bacteria fue altamente pleomórfica y se observaron cambios marcados en el diámetro y la longitud cuando fue subcultivada en medio con agar. La bacteria resultó ser anaeróbica facultativa, capnófila, hemolítica en agar sangre y produjo colonias planas con bordes irregulares después de varios días de incubación. Todas las cepas crecieron en agar de azida sódica pero no en agar MacConkey. Los organismos resultaron ser catalasa y oxidasa negativos y no redujeron el nitrato. Ninguna de las cepas utilizó la arginina, lisina, ornitina o triptófano, pero produjeron ácido a partir de la glucosa, galactosa, levulosa, maltosa, melibiosa, almidón y sucrosa. Todas las cepas produjeron acetoina de la glucosa e hidrolizaron la esculina. Las ocho cepas no pudieron ser identificadas a nivel de género o especie basados en la actual descripción de los organismos clasificados en la división Firmicutes descritos en el Manual de bacteriología sistemática de Bergey.
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ISSN:0005-2086
1938-4351
DOI:10.2307/1591281