Characterization of the plasma-membrane-bound nitrate reductase in Chlorella saccharophila (Kruger) Nadson

• Published in: Planta Vol. 191; no. 1; pp. 79 - 85
• Main Authors: Stohr, C, Tischner, R, Ward, M.R
• Format: Journal Article
• Language: English
• Published: Berlin Springer-Verlag 01-07-1993; Springer
• Subjects: Average linear density; Biological and medical sciences; Cell membranes; Chlorella; Chlorella saccharophila; Chromatography; Detergents; Electrophoresis; enzyme activity; Enzymes; Fundamental and applied biological sciences. Psychology; Gels; Metabolism; nitrate reductase; Nitrates; Plant physiology and development; plasma membrane; Protoplasts; purification; Sodium; Purification; Enzyme; Algae; Chlorophycophyta; Hydrophobicity; Molecular weight; Diagram; Characterization; Membrane enzyme; Nitrate reductase; Polypeptide; Plasma membrane; Oxidoreductases; Thallophyta
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/BF00240898

The plasma membranes of Chlorella saccharophila (Kruger) Nadson cells contained a membrane-bound nitrate reductase. This form of nitrate reductase was purified and characterized. Several differences from the soluble form of nitrate reductase were apparent, the most important being: (i) the greater hydrophobicity, as proven using Triton X-114 phase separation, hydrophobic interaction chromatography and stimulation by phospholipids; (ii) the differences in the native molecular mass compared with Chlorella sorokiniana (Kruger) Nadson; and (iii) the different polypeptide pattern obtained by two-dimensional polyacrylamide gel electrophoresis. Only the plasma-membrane-bound nitrate reductase could be found in both inside-out and right-side-out plasma-membrane vesicles.