CRISPR/Cas9 Genome Editing of the Human Topoisomerase II α Intron 19 5' Splice Site Circumvents Etoposide Resistance in Human Leukemia K562 Cells

An essential function of DNA topoisomerase II (TOP2 ; 170 kDa, TOP2 /170) is to resolve DNA topologic entanglements during chromosome disjunction by introducing transient DNA double-stranded breaks. TOP2 /170 is an important target for DNA damage-stabilizing anticancer drugs, whose clinical efficacy...

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Bibliographic Details

Published in:	Molecular pharmacology Vol. 99; no. 3; pp. 226 - 241
Main Authors:	Hernandez, Victor A, Carvajal-Moreno, Jessika, Papa, Jonathan L, Shkolnikov, Nicholas, Li, Junan, Ozer, Hatice Gulcin, Yalowich, Jack C, Elton, Terry S
Format:	Journal Article
Language:	English
Published:	United States The American Society for Pharmacology and Experimental Therapeutics 01-03-2021
Subjects:	Cell Survival CRISPR-Cas Systems DNA Topoisomerases, Type II - genetics Down-Regulation Drug Resistance, Neoplasm Etoposide - pharmacology Gene Editing - methods Humans Introns K562 Cells Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy Leukemia, Myelogenous, Chronic, BCR-ABL Positive - enzymology Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics Poly-ADP-Ribose Binding Proteins - genetics RNA Splice Sites
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