Effect of sodium selenite on guinea-pig cardiac isolated mitochondria

The pre-incubation with sodium selenite reduces the respiratory index in guinea-pig cardiac mitochondria when alpha-ketoglutarate and glutamate are used as substrates. This decrease is dose-dependent. On the other hand, no modification in respiratory index is measured with malate or malate plus pyru...

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Bibliographic Details
Published in:	Biochemical pharmacology Vol. 35; no. 3; p. 405
Main Authors:	Ignesti, G, Antonini, G, Bennardini, F, Montorsi, L, Dini, G, Franconi, F
Format:	Journal Article
Language:	English
Published:	England 01-02-1986
Subjects:	Animals Coenzyme A - metabolism Glutamates - metabolism Glutamic Acid Guinea Pigs In Vitro Techniques Ketoglutaric Acids - metabolism Malates - metabolism Male Mitochondria, Heart - drug effects Oxygen Consumption - drug effects Pyruvates - metabolism Pyruvic Acid Selenious Acid Selenium - pharmacology
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Summary:	The pre-incubation with sodium selenite reduces the respiratory index in guinea-pig cardiac mitochondria when alpha-ketoglutarate and glutamate are used as substrates. This decrease is dose-dependent. On the other hand, no modification in respiratory index is measured with malate or malate plus pyruvate. The effect on sodium selenite is prevented by CoA administration. Moreover, 25 microM sodium selenite reduces the Km and Vmax of alpha-ketoglutarate dehydrogenase measured at steady-state with variable amount of CoA.
ISSN:	0006-2952
DOI:	10.1016/0006-2952(86)90212-1