Aquimarina versatilis sp. nov., isolated from seashore sand, and emended description of the genus Aquimarina

• Published in: International journal of systematic and evolutionary microbiology Vol. 67; no. 2; pp. 411 - 416
• Main Authors: Lee, Jae Kook, Cha, In-Tae, Kim, Minwook, Choi, Bong-Geun, Song, Hye Seon, Kim, Joon Yong, Roh, Seong Woon, Lee, Sung-Jae
• Format: Journal Article
• Language: English
• Published: England 01-02-2017
• Subjects: Bacterial Typing Techniques; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Flavobacteriaceae - classification; Flavobacteriaceae - genetics; Flavobacteriaceae - isolation & purification; Islands; Phosphatidylethanolamines - chemistry; Phylogeny; Republic of Korea; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Sequence Analysis, DNA; Silicon Dioxide; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.001645

Strain CBA3207T, a novel Gram-stain-negative, aerobic and rod-shaped bacterium, was isolated from the seashore sand of Jeju island in South Korea. Strain CBA3207T grew optimally at 25-30 °C and pH 7.0-7.5 with 3.0-4.0 % (w/v) NaCl. It was catalase-positive, oxidase-negative, and hydrolysed starch, gelatin, and Tweens 20, 40 and 80. The 16S rRNA gene sequence of strain CBA3207T showed 96.0, 95.6, 95.6, 95.5 and 95.5 % similarity to that of Aquimarinamytili PSC33T, Aquimarinaagarivorans HQM9T, Aquimarinalatercula DSM 2041T, Aquimarinaintermedia KMM 6258T and Aquimarinaamphilecti 92VT, respectively. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G and summed feature 9 (10-methyl C16 : 0 and/or iso-C17 : 1ω9c). The major respiratory quinone was menaquinone-6, and the major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and six unidentified lipids. The G+C content of the genomic DNA was 38.8 mol%. Based on phenotypic, genotypic and phylogenetic analyses, strain CBA3207T represents a novel species in the genus Aquimarina, for which the name Aquimarinaversatilissp. nov. is proposed. The type strain is CBA3207T (=KACC 17666T=JCM 19528T).