Paenibacillus zeisoli sp. nov., isolated from maize-cultivated soil artificially contaminated with cadmium

• Published in: International journal of systematic and evolutionary microbiology Vol. 69; no. 4; pp. 1149 - 1154
• Main Authors: Chen, Zhaojin, Ouyang, Wenkai, Chen, Yan, Tian, Wei, Sun, Leni
• Format: Journal Article
• Language: English
• Published: England 01-04-2019
• Subjects: Bacterial Typing Techniques; Base Composition; Cadmium; Cell Wall - chemistry; China; Diaminopimelic Acid - chemistry; DNA, Bacterial - genetics; Fatty Acids - chemistry; Glycolipids - chemistry; Nucleic Acid Hybridization; Paenibacillus - classification; Paenibacillus - isolation & purification; Peptidoglycan - chemistry; Phospholipids - chemistry; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Soil Pollutants; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; Zea mays; China; Paenibacillus genome; Paenibacillus zeisoli; novel species
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.003288

A Gram-stain-negative, rod-shaped, motile bacterial strain, designated 3-5-3
, was isolated from maize-cultivated soil artificially contaminated with cadmium, in Nanyang, Henan Province, China. Strain 3-5-3
was oxidase- and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3
was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2
(96.5 % similarity). The average nucleotide identity and digital DNA-DNA hybridization values between 3-5-3
and the closely related species ranged 69.4-84.5 % and 18.1-18.4 %. The genomic G+C content was 53.8 mol%. Anteiso-C15 : 0 was the major fatty acid and MK-7 was the only menaquinone. The diamino acid in the cell-wall peptidoglycan contains meso-diaminopimelic acid. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids. On the basis of the results obtained in this study, strain 3-5-3
is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacilluszei soli sp. nov. is proposed. The type strain is 3-5-3
(=CGMCC 1.13686
=KCTC 33998
).