Determination of aflatoxins in animal tissues

• Published in: Journal of the Association of Official Analytical Chemists Vol. 64; no. 4; p. 964
• Main Authors: Stubblefield, R D, Shotwell, O L
• Format: Journal Article
• Language: English
• Published: United States 01-07-1981
• Subjects: Aflatoxins - analysis; Animals; Cattle; Chickens; Chromatography - methods; Chromatography, Thin Layer - methods; Densitometry - methods; Humans; Meat - analysis; Swine
• ISSN: 0004-5756
• DOI: 10.1093/jaoac/64.4.964

A method for the determination of aflatoxins in animal tissues has been developed, and applied successfully to beef, swine, chicken, and human livers, and to beef kidney, heart, spleen, muscle, and blood. Blended tissue is denatured with citric acid and extracted with dichloromethane on a wrist-action shaker. After filtration, the extract is partially purified on a silica gel column, and aflatoxins B1 and M1 are determined by 2-dimensional thin layer chromatography and densitometry. Recoveries of B1 and M1 added to meat tissues and blood were approximately 90 and 80%, respectively. The method gave results for a contaminated freeze-dried liver comparable to analyses by 3 other published meat tissue methods. The method is rapid and has a determination limit less than or equal to 0.1 ng/g. In addition, the method uses less toxic and smaller quantities of solvents and chemicals.