Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients

• Published in: Medicina (Kaunas, Lithuania) Vol. 58; no. 5; p. 653
• Main Authors: Kriaučiūnas, Albertas, Liutkevičienė, Rasa, Gedvilaitė, Greta, Kaikarytė, Kristė, Vilkevičiūtė, Alvita, Gleiznys, Darius, Pacauskienė, Ingrida, Žekonis, Gediminas
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 12-05-2022; MDPI
• Subjects: Alleles; Biofilms; Cell cycle; Diabetes; Enzymes; Genes; Genotype & phenotype; Gum disease; Health sciences; Humans; Male; Metabolism; Oral diseases; periodontitis; Periodontitis - genetics; Periodontium; Polymorphism, Single Nucleotide - genetics; Regression analysis; rs3758391; rs3818292; rs7895833; SIRT1; SIRT1 levels; Sirtuin 1 - genetics; Sirtuins - genetics; Statistical analysis; Transplants & implants; United States > US; rs7895833; SIRT1 levels; rs3818292; SIRT1; rs3758391; periodontitis
• ISSN: 1648-9144; 1010-660X; 1648-9144
• DOI: 10.3390/medicina58050653

: Periodontitis is a multifactorial inflammatory disease associated with biofilm dysbiosis and is defined by progressive periodontium destruction. Genes largely regulate this entire process.
s are a group of histone deacetylases (HDACs) intimately involved in cell metabolism and are responsible for altering and regulating numerous cell functions. Understanding
s and their functions in periodontitis may be useful for therapeutic treatment strategies in the future. The aim of our study was to investigate the associations amid
single-gene nucleotide polymorphisms (rs3818292, rs3758391, and rs7895833) and
serum levels for patients affected by periodontitis in the Caucasian population.
: The study included 201 patients affected by periodontitis and 500 healthy controls. DNA extraction from peripheral leukocytes was carried out using commercial kits. The real-time PCR method was selected for the determination of the genotype of the periodontitis patients and the control group. The ELISA method was used to measure the
concentration. A statistical data analysis was performed using "BM SPSS Statistics 27.0" software.
: The
rs3818292 AG genotype was associated with a 2-fold and 1.9-fold increase in the development of periodontitis under the codominant and overdominant models (OR = 1.959; CI = 1.239-3.098;
= 0.004; and OR = 1.944; CI = 1.230-3.073;
= 0.004, respectively). The serum
levels were not statistically significantly different between subjects in the periodontitis and control groups (0.984 (5.159) ng/mL vs. 0.514 (7.705) ng/mL,
= 0.792).
: in our study, the genotypes and alleles of
rs3818292, rs3758391, and rs7895833 statistically significantly differed between the periodontitis and control groups, exclusively in the male population and subjects older than 60 years.