DNA-like duplexes with repetitions. I. Properties of concatemer duplexes formed by d(T-G-C-A-C-A-T-G)

• Published in: Nucleic acids research Vol. 8; no. 11; pp. 2413 - 2430
• Main Authors: Shabarova, Z.A., Dolinnaya, N.G., Turkin, S.I., Gromova, E.S.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 11-06-1980
• Subjects: Carbodiimides; Chemical Phenomena; Chemistry; Circular Dichroism; DNA - chemical synthesis; Macromolecular Substances; Nucleic Acid Conformation; Repetitive Sequences, Nucleic Acid; Spectrophotometry, Ultraviolet; Thermodynamics
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/8.11.2413

A new class of synthetic DHA duplexes containing repeating oligonucleotide sequences, double-helical concatemers, is characterized. The UV-absorption and circular dichroism of a concatemer formed in self-association of d(T-G-C-A-C-A-T-G) have been studied. The thermodynamical parameters of complex formation are the following: ΔH10=-9.2±0.3kcal/molΔS10=-27±1 e.u. The data obtained show that pseudopolymeric duplexes having structures that are similar to MA-B-type helices are formed in solutions of d(T-G-C-A-C-A-T-G) Polymerization of 32P-d(pT-G-C-A-C-A-T-G) induced by water-soluble carbodiimide has been carried out under the conditions of concatemer stability. The yield of the dimer, a 16-member oligonucleotide, was 13%.