Phenolic compounds and bioactive properties of Verbascum glabratum subsp. bosnense (K. Malý) Murb., an endemic plant species

• Published in: Natural product research Vol. 34; no. 16; pp. 2407 - 2411
• Main Authors: Karalija, Erna, Parić, Adisa, Dahija, Sabina, Bešta-Gajević, Renata, Ćavar Zeljković, Sanja
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 17-08-2020; Taylor & Francis Ltd
• Subjects: Agar diffusion test; Antiinfectives and antibacterials; antimicrobial activity; antioxidant activity; Antioxidants; Composition; Endemic plants; Endemic species; Ethanol; Flavonoids; Flowers & plants; p-Hydroxybenzoic acid; Phenolic acids; phenolic compounds; Phenols; Plant species; Polarity; Rosmarinic acid; Salicylic acid; Spectrophotometry; UHPLC-MS/MS; Verbascum; Verbascum glabratum subsp. bosnense (K. Malý) Murb
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2018.1538221

Detailed analysis of phenolic composition and antioxidant and antimicrobial activities of Verbascum glabratum subsp. bosnense (K. Malý) Murb., an endemic species of southeastern Dinaric Alps was performed for the first time. The phenolic composition measured via UHPLC-MS/MS of four extract with different polarity suggested this plant species is very rich in both phenolic acids and flavonoids. Ethanol extract was chemically the most versatile containing 12 compounds with quercitrin and rosmarinic acid as the majors, while water extracts were rich in 4-hydroxybenzoic acid, salicylic acid, morin, and apigenin. All extracts showed high antioxidant potential measured spectrophotometrically with IC
50
values ranging 0.139 - 0.021 mg/mL. Antimicrobial testing using agar diffusion test showed that ethanol extract was the most potent against all tested organisms. Also, these activities are correlated with the content of phenolic compounds, which suggest they are active ingredients of the extracts.