Cloning of a chromosomal alpha-amylase gene from Bacillus stearothermophilus

We have cloned and sequenced a gene for a heat-stable alpha-amylase from a natural isolate of Bacillus stearothermophilus. Previously, it had been shown that B. stearothermophilus amylase genes may be harboured on indigenous plasmids. We have found that our isolate harbours the amylase gene only on...

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Bibliographic Details
Published in:	FEMS microbiology letters Vol. 61; no. 2-3; p. 271
Main Authors:	Jørgensen, P L, Poulsen, G B, Diderichsen, B
Format:	Journal Article
Language:	English
Published:	England 15-01-1991
Subjects:	alpha-Amylases - genetics Amino Acid Sequence Bacillus subtilis - genetics Base Sequence Blotting, Southern Chromosomes, Bacterial Cloning, Molecular - methods DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Escherichia coli - genetics Genes, Bacterial Geobacillus stearothermophilus - enzymology Geobacillus stearothermophilus - genetics Molecular Sequence Data Nucleic Acid Hybridization Plasmids Promoter Regions, Genetic Restriction Mapping
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Summary:	We have cloned and sequenced a gene for a heat-stable alpha-amylase from a natural isolate of Bacillus stearothermophilus. Previously, it had been shown that B. stearothermophilus amylase genes may be harboured on indigenous plasmids. We have found that our isolate harbours the amylase gene only on the chromosome and not on its indigenous plasmid.
ISSN:	0378-1097
DOI:	10.1016/0378-1097(91)90564-Q