Development of a sensitive ELISA for HIV-1 p24 antigen using a fluorogenic substrate for monitoring HIV-1 replication in vitro

A two-site sandwich fluorescent-ELISA was optimized for the detection of HIV-1 p24 antigen produced by lymphoid cells infected with HIV-1 in vitro. To improve the sensitivity of the ELISA, a combination of streptavidin-beta-galactosidase and a fluorogenic substrate (4-methylumbelliferyl-beta-D-galac...

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Bibliographic Details

Published in:	Journal of virological methods Vol. 38; no. 3; p. 323
Main Authors:	Chargelegue, D, O'Toole, C M
Format:	Journal Article
Language:	English
Published:	Netherlands 01-08-1992
Subjects:	Acquired Immunodeficiency Syndrome - diagnosis Antibodies, Monoclonal Dose-Response Relationship, Immunologic Enzyme-Linked Immunosorbent Assay - methods HIV Core Protein p24 - analysis HIV-1 - physiology Humans In Vitro Techniques Sensitivity and Specificity Time Factors Virus Replication
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