Bioprospecting for electrochemically active perchlorate-reducing microorganisms

• Published in: Bioelectrochemistry (Amsterdam, Netherlands) Vol. 147; p. 108171
• Main Authors: Torres-Rojas, Felipe, Muñoz, Diana, Pía Canales, Camila, Vargas, Ignacio T.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-10-2022; Elsevier BV
• Subjects: Biocathode; Bioelectrochemical system; Bioprospecting; Chlorate; Electrochemical analysis; Electrochemical cells; Electrochemical impedance spectroscopy; Electrochemically active microorganisms; Electrochemistry; Microorganisms; Perchlorate; Perchloric acid; Reduction; Silver chloride; Spectroscopy; Perchlorate; Biocathode; Electrochemically active microorganisms; Bioelectrochemical system
• ISSN: 1567-5394; 1878-562X
• DOI: 10.1016/j.bioelechem.2022.108171

This study evaluated the electrochemical capacity of four perchlorate-reducing microorganisms (PRMs) isolated from an Altiplanic Andean watershed naturally pressured with perchlorate. Three-electrode electrochemical cells were used to test the electrochemical activity of the obtained isolates. Electrochemical evaluation (i.e., cyclic voltammetry, electrochemical impedance spectroscopy, chronoamperometry) revealed that two isolates identified as Dechloromonas sp. CS-1 and Clostridioides sp. CS-2 are electrochemically active PRMs. Bacterial isolates exhibiting cathodic peaks at -651 mV and -303 mV (vs. Ag/AgCl) for CS-1 and CS-2, respectively. Electrotrophic perchlorate removal was demonstrated by a 6-days chronoamperometry with removal rates of 27 and 17 mg L-1 day-1 and cathodic efficiencies of 93% and 45%, for CS-1 and CS-2, respectively. Chemical and electrochemical results suggest two different mechanisms of electrotrophic perchlorate removal, a complete eight-electron bio-reduction (i.e., perchlorate to chloride) for CS-1 and a partial two-electron bio-reduction (i.e., perchlorate to chlorate) for CS-2. The observed differences could be linked to their enzymatic differences, as in their membrane compositions. Thus, the results of this work increase the limited number of known electrotrophic microorganisms and expand the application of bioelectrochemical systems to develop new perchlorate treatment and remediation technologies.