New horizons of genomic in situ hybridization

This review summarizes conventional and recent applications of genomic in situ hybridization (GISH). GISH is a well recognized technique, but its modifications and applications have not been widely used. Here, we show how modifications to the GISH technique can be used as tools to 'paint'...

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Bibliographic Details
Published in:	Cytogenetic and genome research Vol. 126; no. 4; p. 368
Main Authors:	Markova, M, Vyskot, B
Format:	Journal Article
Language:	English
Published:	Switzerland 01-01-2009
Subjects:	Animals Base Sequence Chromosomes, Plant Humans In Situ Hybridization - methods Karyotyping Phylogeny
Online Access:	Get more information
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Summary:	This review summarizes conventional and recent applications of genomic in situ hybridization (GISH). GISH is a well recognized technique, but its modifications and applications have not been widely used. Here, we show how modifications to the GISH technique can be used as tools to 'paint' plant chromosomes. In addition, we describe novel applications, e.g. how GISH banding could be used for karyotyping plant chromosomes. We further discuss recent phylogenetic applications of GISH that allow a semiquantitative signal analysis and the possibility of comparing and combining this cytogenetic technique with DNA sequence-based phylogenetic trees.
ISSN:	1424-859X
DOI:	10.1159/000275796