Co‐delivery of tauroursodeoxycholic acid and dexamethasone using electrospun ultrafine fibers to induce early coupled angiogenesis and osteogenic differentiation

• Published in: Journal of applied polymer science Vol. 139; no. 47
• Main Authors: Liu, Junyu, Tan, Ziwei, Jia, Yongliang, Shi, Xiaotong, Hou, Ruxia, Liu, Jiajia, Luo, Dongmei, Fu, Xinyu, Yang, Tingting, Wang, Xiangyu
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 15-12-2022; Wiley Subscription Services, Inc
• Subjects: Alkaline phosphatase; Angiogenesis; angiogenic activity; bone; Dexamethasone; Differentiation (biology); dual drug delivery; electrospinning; Materials science; Mechanical properties; osteogenic activity; Polycaprolactone; Polymers; Regeneration (physiology); Scaffolds; Ultrafines; Wound healing
• ISSN: 0021-8995; 1097-4628
• DOI: 10.1002/app.53173

In the complicated process of bone regeneration, osteogenic differentiation and angiogenesis are crucial. In this study, a novel dual small molecule composite electrospun scaffold was prepared using polycaprolactone embedded with angiogenic factor tauro‐ursodeoxycholic acid and osteogenic factor dexamethasone to promote early angiogenesis and osteogenesis. Additionally, the related properties, angiogenic activity, and osteogenic activity analyses were conducted for the scaffold. The results demonstrated that the composite scaffold possessed proper mechanical properties and hydrophilicity. During the initial drug release, the release rate of the hydrophilic drug tauro‐ursodeoxycholic acid was higher compared to the hydrophobic drug dexamethasonethe. The drugs were slowly sustained released for more than 528 h. The composite scaffold boosted cell proliferation and accelerated osteogenic differentiation up to 21 days. The alkaline phosphatase activity and mineral deposition were comparatively higher on dual drug‐releasing fibers compared to control scaffolds. Wound healing migration assay and tube formation assay for further in vitro revealed that the composite scaffold exhibited higher efficiency in promoting cell migration and the angiogenesis ductive. Hence, the composite scaffold had a high potential for promoting bone regeneration by enhancing angiogenesis.
A novel dual small molecule composite electrospun scaffold is prepared using polycaprolactone (PCL) embedded with angiogenic factor tauro‐ursodeoxycholic acid (TUDCA) and osteogenic factor Dexamethasone (DEX). The sequential release of drugs in the composite scaffold facilitates early induction of angiogenesis and continuous induction of bone regeneration, and this composite scaffold could potentially be used to promote bone tissue regeneration.