Genetic relationships among strains of Xylella fastidiosa from RAPD-PCR data

• Published in: Current microbiology Vol. 31; no. 2; pp. 134 - 137
• Main Authors: Pooler, M.R. (Fruit Laboratory, ARS, USDA, Beltsville Agricultural Research Center, Beltsville, MD.), Hartung, J.S
• Format: Journal Article
• Language: English
• Published: New York, NY Springer 01-08-1995
• Subjects: ADN; AMBROSIA; AMPLIFICATION CHAINE POLYMERASE; BACTERIA; Bacterial plant pathogens; Bacteriology; Base Sequence; Biological and medical sciences; Biotechnology; CITRUS; DISTANCE GENETIQUE; DISTANCIA GENETICA; DNA Primers; FILOGENIA; Fundamental and applied biological sciences. Psychology; Genetics; Gram-Negative Bacteria - classification; Gram-Negative Bacteria - genetics; HOTE; HUESPEDES; Microbiology; Molecular Sequence Data; MORUS; PHYLOGENIE; Phylogeny; Phytopathology. Animal pests. Plant and forest protection; POLIMORFISMO; Polymerase Chain Reaction - methods; POLYMORPHISME; PROCEDENCIA; PROVENANCE; PRUNUS; REACCION DE CADENAS DE POLIMERASA; Species Specificity; Systematics. Structure, properties and multiplication. Genetics; ULMUS; VITIS; Polymerase chain reaction; Plant pathogen; Bacteria; Genotype; Genetic distance; Comparative study; Strain
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/BF00294290

Genetic relationships among 11 Xylella fastidiosa strains isolated from mulberry, almond, ragweed, grape, plum, elm, and citrus were determined by random amplified polymorphic DNA (RAPD). Twenty-two 10-base primers amplified a total of 77 discrete polymorphic bands. Phenetic analysis based on a similarity matrix corresponded well with previous reports on X. fastidiosa RFLP-based similarity relationships, indicating that RAPD-PCR amplification products can be used as a reliable indicator of genetic distance in X. fastidiosa. Cladistic analysis suggests the existence of five groups of X. fastidiosa: the citrus group, the plum-elm group, the grape-ragweed group, the almond group, and the mulberry group