Raman spectroscopy as a novel method in placental research: Recognizing the pattern of placental hypoxia

• Published in: Journal of Raman spectroscopy Vol. 48; no. 12; pp. 1896 - 1899
• Main Authors: Schlabritz‐Loutsevitch, Natalia, Gandhi, Kushal, Soydemir, Fatimah, Brownbill, Paul, Sengar, Raghvendra, Ventolini, Gary, Bruillard, Paul, Gosink, Luke
• Format: Journal Article
• Language: English
• Published: Bognor Regis Wiley Subscription Services, Inc 01-12-2017
• Subjects: Feasibility studies; Fetuses; Hypoxia; Pattern recognition; Perfusion; Placenta; Raman spectroscopy; Spectroscopy; Spectrum analysis
• ISSN: 0377-0486; 1097-4555
• DOI: 10.1002/jrs.5229

Raman spectroscopy (RS) is a nonelastic photon scattering technique. We evaluated whether a specific RS pattern exists in fetal venous perfusates obtained at 30‐ to 60‐min intervals from the ex vivo human dual placental perfusion model under hypoxic (n = 6) and normoxic (n = 5) conditions. A stratified principle component and a linear support vector machine analyses showed that the separation between 2 conditions was readily feasible at 60–120 min of perfusion; after this, any apparent differences were most likely the result of artifact overfitting. This report is the first attempt to identify the RS fingerprint associated with placental hypoxia.
Raman spectroscopy (RS) is a nonelastic photon scattering technique. We evaluated whether a specific RS pattern exists in fetal venous perfusates obtained at 30‐min intervals from the ex vivo human dual placental perfusion model under hypoxic (n = 6) and normoxic (n = 5) conditions, a stratified principle component and a linear support vector machine analyses showed that the separation between two conditions was readily feasible at 60–120 min of perfusion; after this, any apparent differences were most likely the result of artifact overfitting.