Determination of ophidine in human urine

Ophidine (beta-alanyl-3-methylhistidine) was first detected in the urine of two patients and later in two members of the laboratory staff loaded with whale meat, by column chromatography, high-voltage paper electrophoresis and two-dimensional paper chromatography. The ophidine peak was detected betw...

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Bibliographic Details
Published in:	Journal of chromatography Vol. 227; no. 1; p. 53
Main Authors:	Undrum, T, Lunde, H, Gjessing, L R
Format:	Journal Article
Language:	English
Published:	Netherlands 01-01-1982
Subjects:	Animals Anserine - urine Cats Chromatography, Paper Diet Dipeptides - urine Dolphins Electrophoresis, Paper Horses Humans Meat Muscles - analysis Poultry Rabbits Reptiles Whales
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Summary:	Ophidine (beta-alanyl-3-methylhistidine) was first detected in the urine of two patients and later in two members of the laboratory staff loaded with whale meat, by column chromatography, high-voltage paper electrophoresis and two-dimensional paper chromatography. The ophidine peak was detected between homocarnosine and dimethylarginine using a lithium buffer gradient in column chromatography. In paper chromatography the ophidine spot was detected at a position close to anserine and homocarnosine. The ophidine in the urine from the patients was of dietary origin since it was absent in the urine a few weeks later.
DOI:	10.1016/S0378-4347(00)80355-2