Determination of polyamines in human erythrocytes by capillary gas chromatography with nitrogen-phosphorus detection

Determination of polyamines in human erythrocytes by capillary gas chromatography with nitrogen-phosphorus detection

A capillary gas chromatographic method with nitrogen-phosphorus detection is used to determine simultaneously 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine in human erythrocytes. The compounds are isolated by adsorption on silica and converted into their heptafluorobutyryl deri...

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Bibliographic Details
Published in:Journal of chromatography Vol. 415; no. 1; p. 27
Main Authors: van den Berg, G A, Kingma, A W, Muskiet, F A
Format: Journal Article
Language:English
Published: Netherlands 1987
Subjects:
Adult
Chromatography, Gas
Erythrocytes - analysis
Humans
Indicators and Reagents
Male
Polyamines - blood
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Summary:A capillary gas chromatographic method with nitrogen-phosphorus detection is used to determine simultaneously 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine in human erythrocytes. The compounds are isolated by adsorption on silica and converted into their heptafluorobutyryl derivatives. We give quality-control data and (age-dependent) normal values for 48 apparently healthy controls.
DOI:10.1016/S0378-4347(00)83188-6