Comparative Analysis of Gut Microbial Communities in Children under 5 Years Old with Diarrhea

• Published in: Journal of microbiology and biotechnology Vol. 28; no. 4; pp. 652 - 662
• Main Authors: Wen, Hongyu, Yin, Xin, Yuan, Zhenya, Wang, Xiuying, Su, Siting
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 28-04-2018
• Subjects: Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Biomarkers; Child, Preschool; Diarrhea - complications; Diarrhea - diagnosis; Diarrhea - microbiology; DNA, Bacterial - genetics; Enterococcus - genetics; Feces - microbiology; Female; Gastrointestinal Microbiome - genetics; High-Throughput Nucleotide Sequencing - methods; Humans; Infant; Infant, Newborn; Male; Microbial Consortia - genetics; Phylogeny; RNA, Ribosomal, 16S - genetics; 생물학; Children diarrhea; high-throughput sequencing; 16S rRNA genes; gut microbial communities
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1711.11065

Diarrhea is a global disease with a high morbidity and mortality rate in children. In this study, 25 fecal samples were collected from children under 5 years old. Seven samples had been taken from healthy children without diarrhea and marked as the healthy control group; eight samples had been sampled from children with diarrhea caused by dyspepsia and defined as the non-infectious group; and ten samples had been taken from children with diarrhea induced by intestinal infections and identified as the infectious group. We detected the microbial communities of samples by using high-throughput sequencing of 16S rRNA genes. The proportion of aerobic and facultative anaerobic microbes in samples of the infectious group was much higher than in the non-infectious group. In addition, the relative abundance of
in the healthy control group was significantly higher than in the non-infectious group and infectious group. This can be used as a potential diagnostic biomarker for diarrhea.