Photooxidation of Nanopatterned Poly(chloromethylstyrene): Direct Formation of Crosslinked Aldehyde-Functionalized Films for Chemical Functionalization and Bioconjugation

• Published in: Macromolecular rapid communications. Vol. 31; no. 9-10; pp. 910 - 914
• Main Authors: Yu, Xi, Subramani, Chandramouleeswaran, Yang, Xiaochao, Kim, Chae Kyu, Rotello, Vincent M.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 12-05-2010; WILEY‐VCH Verlag
• Subjects: Aldehydes; Crosslinking; enzyme immobilization; Enzymes; Nanocomposites; nanoimprint lithography; Nanomaterials; nanoparticles; Nanostructure; polymers; Surface chemistry; surface functionalization; surface patterning; X-ray photoelectron spectroscopy
• ISSN: 1022-1336; 1521-3927
• DOI: 10.1002/marc.200900925

UV irradiation of chloromethyl styrene simultaneously crosslinks the polymer and generates aldehyde groups that can be employed for polymer surface functionalization through aldehyde‐imine/hydrazone chemistry. Using this method, we successfully have functionalized nanoimprinted polymer patterns with dyes, nanoparticles, and enzymes. These surfaces were characterized by infrared (ATR‐IR) spectroscopy, X‐ray photoelectron spectroscopy (XPS), fluorescence microscopy, and enzymatic activity assays.
Aldehyde groups generated upon UV irradiation of chloromethyl styrene are utilized as surface active groups for polymer surface functionalization through aldehyde‐amine chemistry. Using this method, we successfully activate the inert nanoimprint polymer resist pattern, allowing facile immobilization of dyes, nanoparticles, and enzymes onto the pattern. The processes were characterized by attenuated total reflection infrared (ATR‐IR), X‐ray photoelectron spectroscopy (XPS), and fluorescence microscopy.