Comparison of immediate polyvinyl alcohol (PVA) fixation with delayed Schaudinn's fixation for the demonstration of protozoa in stool specimens

Comparison of immediate polyvinyl alcohol (PVA) fixation with delayed Schaudinn's fixation for the demonstration of protozoa in stool specimens

Immediate fixation of stool specimens in polyvinyl alcohol fixative (PVA) was compared with Schaudinn's fixation delayed until the specimens were received in the laboratory, in a series of 100 consecutive positive stool specimens. More specimens were found positive following PVA fixation, and t...

Full description

Saved in:
Bibliographic Details
Published in:The American journal of tropical medicine and hygiene Vol. 26; no. 6 Pt 1; p. 1139
Main Authors: Markell, E K, Quinn, P M
Format: Journal Article
Language:English
Published: United States 01-11-1977
Subjects:
Amebiasis - parasitology
Dientamoebiasis - parasitology
Endolimax
Entamoebiasis - parasitology
Eukaryota
Feces - parasitology
Fixatives
Giardiasis - parasitology
Humans
Methods
Polyvinyl Alcohol
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Immediate fixation of stool specimens in polyvinyl alcohol fixative (PVA) was compared with Schaudinn's fixation delayed until the specimens were received in the laboratory, in a series of 100 consecutive positive stool specimens. More specimens were found positive following PVA fixation, and the numbers of organisms present on the slides were greater in specimens processed by this technique than after Schaudinn's fixation. It is concluded that immediate fixation results in the preservation of larger numbers of organisms in a recognizable state. The routine use of PVA fixation prior to transportation of the specimen to the laboratory is recommended.
ISSN:0002-9637
DOI:10.4269/ajtmh.1977.26.1139