Cognitive impairment related changes in the elemental concentration in the brain of old rat

• Published in: Spectrochimica acta. Part B: Atomic spectroscopy Vol. 61; no. 10; pp. 1219 - 1223
• Main Authors: Serpa, R.F.B., de Jesus, E.F.O., Anjos, M.J., Lopes, R.T., do Carmo, M.G.T., Rocha, M.S., Rodrigues, L.C., Moreira, S., Martinez, A.M.B.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-11-2006
• Subjects: Brain; Learning; Memory; SR-TXRF; Trace element; Learning; Brain; Trace element; SR-TXRF; Memory
• ISSN: 0584-8547; 1873-3565
• DOI: 10.1016/j.sab.2006.08.005

In order to evaluate the elemental concentration as a function of learning and memory deficiency, six different structures of the brain were analyzed by total reflection X-ray fluorescence spectrometry with synchrotron radiation (SR-TXRF). To evaluate the cognitive processes, the animals were tested in an adaptation of the Morris water maze. After the test, the animals were divided into two groups: cognitively healthy (control group) and cognitively impaired. The measurements were carried out at XRF beam line at Light Synchrotron Brazilian laboratory, Campinas, Brazil. The following elements were identified: Al, P, S, Cl, K, Ca, Ti, Cr, Fe, Cu, Zn, Br and Rb. K concentration was higher in all regions of the brain studied for control group than the cognitively impaired group. Moreover, the control group presented higher levels for P and Fe in the entorhinal cortex, in the temporal cortex (only P), in the hypothalamus and in the thalamus, than the cognitively impaired group. Br concentration in the animals which presented cognitive impairment was three times larger in the hypothalamus and thalamus, twice larger in temporal cortex and higher in visual cortex than the cognitively healthy group. Cu was more remarkable in the hippocampus and hypothalamus from the animals with cognitive impairment than the control group.
We observed that the cognitively impaired group presented highest concentrations of Br and Cu in certain areas than the control group, on the other hand, this group presented highest levels of K for all brain areas studied.