Influence of host lineage on cecal colonization by Campylobacter jejuni in chickens

• Published in: Avian diseases Vol. 34; no. 3; pp. 602 - 606
• Main Authors: Stern, Norman J., Meinersmann, Richard J., Cox, Nelson A., Bailey, J. Stan, Blankenship, Leroy C.
• Format: Journal Article
• Language: English
• Published: United States American Association of Avian Pathologists, Inc 01-07-1990
• Subjects: Animals; Birds; broiler chickens; Campylobacter; Campylobacter Infections - genetics; Campylobacter Infections - immunology; Campylobacter Infections - veterinary; Campylobacter jejuni; Campylobacter jejuni - growth & development; Campylobacter jejuni - immunology; Carrier State - immunology; Carrier State - veterinary; Cecum - microbiology; Chickens; Crosses, Genetic; descendance; disease resistance; enfermedades transm por alimentos; enterite; enteritis; Flocks; foodborne diseases; genetica; genetics; genetique; Immune response; Immunity, Innate - genetics; intestin; intestines; intestinos; Jejunal diseases; maladie transmissible par aliment; Medical genetics; Microbial colonization; pollo de engorde; poulet de chair; Poultry Diseases - genetics; Poultry Diseases - immunology; progenie; progeny; Receptors; reponse immunitaire; resistance aux maladies; resistencia a la enfermedad; respuesta inmunologica
• ISSN: 0005-2086; 1938-4351
• DOI: 10.2307/1591251

The resistance to cecal colonization by Campylobacter jejuni was assessed by challenging three crossbred stocks of commercially available broiler chickens. These three stocks, designated A, B, and C, were related as follows: Offspring from four pedigreed grandparent flocks were used as progenitors. Stock B was derived by cross-breeding grandparent 1 with grandparent 3. Stocks A and C were crossbreeds from grandparents 1 and 2 and grandparents 3 and 4, respectively. Campylobacter jejuni were gavaged into 48-hour-old chicks, using the same levels of challenge dose for each of the different chicken stocks. Six days post-challenge, the birds were sacrificed, and cecal contents were plated onto Campylobacter-selective media. Results from two replicate trials with three isolates of C. jejuni indicated that chicken stock A was colonized in only two of 60 ceca, stock B in six of 60, and stock C in 19 of 60 chicken ceca. Statistical analysis of these data indicate that resistance to cecal colonization by C. jejuni was significantly (P < 0.05) influenced through chicken host lineage. /// La resistencia a la colonización cecal del Campylobacter jejuni fue evaluada mediante el desafío de tres líneas comerciales de pollos de engorde. Estas tres líneas, designadas A, B y C, estaban relacionadas como sigue: las líneas A y C eran primas de la línea B, pero no estaban relacionadas entre ellas. El C. jejuni fue administrado por sonda esofágica a pollos de 48 horas de edad, usando el mismo nivel de dosis de desafío para cada una de las diferentes líneas comerciales. Seis días después del desafío, las aves fueron sacrificadas y el contenido cecal se cultivó en medio selectivo para Campylobacter. Los resultados de dos experimentos replicados usando tres cepas de C. jejuni indicaron que en la línea A hubo colonización en sólo 2 de 60 ciegos, en la línea B en 6 de 60 y en la línea C en 19 de 60 ciegos. El análisis estadístico de estos datos indica que la resistencia a la colonización cecal por el C. jejuni puede ser influenciada significativamente (P < 0.05) por la línea comercial del huésped aviar.