Determination of testosterone in human hair by gas chromatography-selected ion monitoring mass spectrometry
A method for the determination of testosterone in human hair by gas chromatography-mass spectrometry using d3-testosterone as internal standard is described. Our method consisted of alkaline digestion, fast liquid-liquid extraction, LH-20 chromatography and derivatization with heptafluorobutyric anh...
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| Published in: | Analyst (London) Vol. 123; no. 12; p. 2661 |
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| Main Authors: | , , |
| Format: | Journal Article |
| Language: | English |
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England
01-12-1998
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| Abstract | A method for the determination of testosterone in human hair by gas chromatography-mass spectrometry using d3-testosterone as internal standard is described. Our method consisted of alkaline digestion, fast liquid-liquid extraction, LH-20 chromatography and derivatization with heptafluorobutyric anhydride. Quantification was achieved by selected ion monitoring of m/z 680 (testosterone) and m/z 683 (d3-testosterone). Our method needed no complex corrections for isotope contributions. The procedure provided a sensitive and specific technique with good accuracy and precision. For the first time, testosterone has been quantified by gas chromatography-mass spectrometry in human hair. The concentrations (median, range, ng g-1 hair) reflected a significant (p = 0.05; t-test) sex difference with 2.7 ng g-1 (2.5-4.2) in male and 1.7 ng g-1 (1.0-3.4) in female hair. |
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| AbstractList | A method for the determination of testosterone in human hair by gas chromatography-mass spectrometry using d3-testosterone as internal standard is described. Our method consisted of alkaline digestion, fast liquid-liquid extraction, LH-20 chromatography and derivatization with heptafluorobutyric anhydride. Quantification was achieved by selected ion monitoring of m/z 680 (testosterone) and m/z 683 (d3-testosterone). Our method needed no complex corrections for isotope contributions. The procedure provided a sensitive and specific technique with good accuracy and precision. For the first time, testosterone has been quantified by gas chromatography-mass spectrometry in human hair. The concentrations (median, range, ng g-1 hair) reflected a significant (p = 0.05; t-test) sex difference with 2.7 ng g-1 (2.5-4.2) in male and 1.7 ng g-1 (1.0-3.4) in female hair. |
| Author | Wudy, S A Wachter, U Scherer, C |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/10435320$$D View this record in MEDLINE/PubMed |
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| SubjectTerms | Female Gas Chromatography-Mass Spectrometry Hair - chemistry Humans Male Sensitivity and Specificity Substance Abuse Detection Testosterone - analysis |
| Title | Determination of testosterone in human hair by gas chromatography-selected ion monitoring mass spectrometry |
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