Prostaglandins from human T suppressor/cytotoxic cells modulate natural killer antibacterial activity

We have recently described potent antibacterial activity of purified human NK cells. Here we show that this function is regulated by T cytotoxic/suppressor CD8+ cells. Thus, coculture of NK and CD8+ cells for 3 h or longer times abrogated the expression of the NK antibacterial activity, and of two a...

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Published in:	The Journal of experimental medicine Vol. 170; no. 2; pp. 601 - 606
Main Authors:	Garcia-Peñarrubia, P, Bankhurst, A D, Koster, F T
Format:	Journal Article
Language:	English
Published:	United States The Rockefeller University Press 01-08-1989
Subjects:	Antigens, Differentiation, T-Lymphocyte - analysis Blood Bactericidal Activity CD8 Antigens Cell Separation Dinoprostone - physiology Humans In Vitro Techniques Indomethacin - pharmacology Killer Cells, Natural - immunology T-Lymphocytes, Cytotoxic - immunology T-Lymphocytes, Regulatory - immunology
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Abstract	We have recently described potent antibacterial activity of purified human NK cells. Here we show that this function is regulated by T cytotoxic/suppressor CD8+ cells. Thus, coculture of NK and CD8+ cells for 3 h or longer times abrogated the expression of the NK antibacterial activity, and of two activation markers IL-2R and transferrin receptor (Tf-R). The suppressive activity was mediated by PGE2 as demonstrated by direct PGE2 determination in CD8+ cell free supernatants, and by inhibition of CD8+ cell suppression with indomethacin or piroxicam in vitro. We also found that resting T cytotoxic/suppressor cells purified by negative selection produce higher amounts of PGE2 than adherent cells like monocytes and macrophages, and that these concentration levels are in the range of concentrations known to suppress a significant number of in vitro immunologic functions.
AbstractList	We have recently described potent antibacterial activity of purified human NK cells. Here we show that this function is regulated by T cytotoxic/suppressor CD8+ cells. Thus, coculture of NK and CD8+ cells for 3 h or longer times abrogated the expression of the NK antibacterial activity, and of two activation markers IL-2R and transferrin receptor (Tf-R). The suppressive activity was mediated by PGE2 as demonstrated by direct PGE2 determination in CD8+ cell free supernatants, and by inhibition of CD8+ cell suppression with indomethacin or piroxicam in vitro. We also found that resting T cytotoxic/suppressor cells purified by negative selection produce higher amounts of PGE2 than adherent cells like monocytes and macrophages, and that these concentration levels are in the range of concentrations known to suppress a significant number of in vitro immunologic functions. The authors have recently described potent antibacterial activity of purified human NK cells. Here they show that this function is regulated by T cytotoxic/suppressor CD8 super(+) cells. Thus, coculture of NK and CD8 super(+) cells for 3 h or longer times abrogated the expression of the NK antibacterial activity, and of two activation markers IL-2R and transferrin receptor (Tf-R). The suppressive activity was mediated by PGE sub(2) as demonstrated by direct PGE sub(2) determination in CD8 super(+) cell free supernatants, and by inhibition of CD8 super(+) cell suppression with indomethacin or piroxicam in vitro. The authors also found that resting T cytotoxic/suppressor cells purified by negative selection produce higher amounts of PGE sub(2) than adherent cells like monocytes and macrophages, and that these concentration levels are in the range of concentrations known to suppress a significant number of in vitro immunologic functions.
Author	Garcia-Peñarrubia, P Koster, F T Bankhurst, A D
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Snippet	We have recently described potent antibacterial activity of purified human NK cells. Here we show that this function is regulated by T cytotoxic/suppressor... The authors have recently described potent antibacterial activity of purified human NK cells. Here they show that this function is regulated by T...
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SubjectTerms	Antigens, Differentiation, T-Lymphocyte - analysis Blood Bactericidal Activity CD8 Antigens Cell Separation Dinoprostone - physiology Humans In Vitro Techniques Indomethacin - pharmacology Killer Cells, Natural - immunology T-Lymphocytes, Cytotoxic - immunology T-Lymphocytes, Regulatory - immunology
Title	Prostaglandins from human T suppressor/cytotoxic cells modulate natural killer antibacterial activity
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