Development and Validation of Improved Reversed Phase-HPLC Method for Simultaneous Determination of Curcumin, Demethoxycurcumin and Bis-Demethoxycurcumin

• Published in: Chromatographia Vol. 65; no. 7-8; pp. 483 - 488
• Main Authors: Jadhav, B.-K, Mahadik, K.-R, Paradkar, A.-R
• Format: Journal Article
• Language: English
• Published: Oxford Wiesbaden : Vieweg Verlag 01-04-2007; Springer; Springer Nature B.V
• Subjects: Acetic acid; Acetonitrile; Aroma and flavouring agent industries; Biological and medical sciences; Curcumin, demethoxycurcumin, and bis-demethoxycurcumin; Flow velocity; Food industries; Fundamental and applied biological sciences. Psychology; General pharmacology; High performance liquid chromatography; Ions; liquid chromatography; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Reversed phase-HPLC; Turmeric rhizome extract; Asia; China; India; Monocotyledones; Chemical analysis; HPLC chromatography; Medicinal plant; Organic pigment; Column liquid chromotography; Folk medicine; Angiospermae; Ayurvedic medicine; Rhizome; Curcumin; Quantitative analysis; Below ground plant part; Validation; and bis-demethoxycurcumin; Natural dye; Pharmacognosy; Visible spectrometry; Isocratic condition; Reversed phase-HPLC; demethoxycurcumin; Turmeric; Reversed phase chromatography; Dye plant; Simultaneous measurement; Plant origin; Turmeric rhizome extract; Flavoring crop; Spermatophyta; Zingiberaceae
• ISSN: 0009-5893; 1612-1112
• DOI: 10.1365/s10337-006-0164-8

Isocratic reversed phase high performance liquid chromatographic (HPLC) method using RP C18 column was developed for simultaneous determination of the curcuminoids. Mobile phase consisted of acetonitrile:0.1% trifluro-acetic acid (50:50) and flow rate was 1.5 mL min-¹ and elution was monitored at 420 nm. Validation in selected conditions showed that the chosen method is sensitive, selective, precise and reproducible with linear response of detector for the simultaneous determination of curcumin (C), demethoxycurcumin (DMC) and bis-demethoxycurcumin (BDMC). The limits of detection were 27.99, 31.91 and 21.81 ng mL-¹ for C, DMC and BDMC, respectively. Limits of quantitation for C, DMC and BDMC, were 84.84, 96.72 and 66.10 ng mL-¹, respectively. Linear range was form 100 to 600 ng mL-¹. The mean ± SD percent recoveries of curcuminoids were 99.87 ± 0.34, 100.09 ± 0.48 and 100.10 ± 0.60% of C, DMC and BDMC, respectively. Further, the method was used for quantitation of curcuminoids from turmeric rhizome.