Study of antifungal agent caspofungin adsorption to laboratory materials

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1188; p. 123060
• Main Authors: Uribe, B., Yaldebere, A., González, O., Guruceaga, X., Ramirez-Garcia, A., Rementeria, A., Ba, B.B., Gaudin, K., Alonso, R.M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-01-2022
• Subjects: Adsorption; Antifungal; Antifungal Agents - analysis; Antifungal Agents - chemistry; Antifungal Agents - metabolism; Caspofungin; Caspofungin - analysis; Caspofungin - chemistry; Caspofungin - metabolism; Chromatography, High Pressure Liquid - instrumentation; Glass - chemistry; HPLC; Materials; MIC; Microbial Sensitivity Tests; Plastics - chemistry; Plastics - metabolism; Antifungal; Adsorption; MIC; HPLC; Caspofungin; Materials
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2021.123060

•Adsorption of caspofungin on plastic materials, questions results obtained in vitro.•The caspofungin adsorption occurs on various materials such as plastic and glassware.•Effect of container, pH and organic solvent was studied on caspofungin adsorption.•The use of acidic pH avoids adsorption of caspofungin on glassware material.•Addition of organic solvent avoids adsorption of caspofungin on glassware material.
Treatment of invasive fungal infections with Caspofungin is used as the first-line antifungal agents. The minimum inhibitory concentration value is a test which indicates the degree of sensitivity of a strain regarding a drug. However, no value of minimum inhibitory concentration for caspofungin is available because very variable value is obtained. In this work, we study the link with the adsorption phenomenon of CSF previously described in literature and the lack of minimum inhibitory concentration value.
A systematic study of the impact of different parameters on CSF adsorption is reported. The effect of the nature of container material, the aqueous solution pH and the organic solvent proportion was studied. In addition, the possibility of using a coating agent to minimize the adsorption was assayed and evaluated.
Results obtained showed the importance of the material used during the manipulation of CSF. The use of acidic pH aqueous solution or the addition of acetonitrile or methanol proportions (50 % and 70 %, respectively) were found efficient to avoid adsorption of CSF on glassware material, which is the relevant strategy for analytical samples of caspofungin. The treatment of HPLC glass vials and 96-well plates with N-(2-aminoethyl)-3-aminopropyltrimethoxysilane reduced the adsorption. The significant adsorption observed in this work especially with plastic materials, questions the results obtained before in different assays and explained the absence of MIC value.