Preparing compound heterozygous reference material using gene synthesis technology: a model of thrombophilic mutations

The aim of our study is to present a novel approach for preparing a compound heterozygous reference material (hetRM) using gene synthesis technology with inverted insertion of wild-type and mutant fragments into a single cloning vector. Factor II (G20210A) and Factor V (G1691A Leiden) gene mutations...

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Bibliographic Details

Published in:	Biomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia Vol. 158; no. 4; pp. 539 - 543
Main Authors:	Beranek, Martin, Drastikova, Monika, Dulicek, Petr, Palicka, Vladimir
Format:	Journal Article
Language:	English
Published:	Czech Republic 01-12-2014
Subjects:	Cloning, Organism - methods Escherichia coli Factor V - genetics Genetic Techniques Mutation Prothrombin - genetics Real-Time Polymerase Chain Reaction Sequence Analysis, DNA Thrombophilia - genetics
Online Access:	Get full text
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