Molecular characterization of Leishmania infantum isolates in municipalities of the state of Minas Gerais, Brazil

• Published in: Transactions of the Royal Society of Tropical Medicine and Hygiene Vol. 112; no. 1; pp. 43 - 45
• Main Authors: Nunes, Ramon Vieira, Quaresma, Patrícia Flávia, Rugani, Jeronimo Marteleto Nunes, Teixeira-Neto, Rafael Gonçalves, Saboia Vahia, Leonardo, da Silva, Eduardo Sérgio
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01-01-2018
• Subjects: hsp70; Molecular markers; ITS1; mpi; Polymorphism
• ISSN: 0035-9203; 1878-3503
• DOI: 10.1093/trstmh/try018

Abstract
Background
Little is known about the genetic diversity of Leishmania infantum isolates in the state of Minas Gerais, Brazil.
Methods
Molecular characterization of hsp70, mpi and ITS1 was carried out for 29 isolates of L. infantum from the bone marrow of naturally infected dogs from the cities of Divinópolis, Pará de Minas and Brumadinho, located in the central-west, central and central regions of the state of Minas Gerais, Brazil, respectively.
Results
Analysis of the parasite nucleotide sequences demonstrated very high homogeneity of the studied samples.
Conclusions
In the endemic regions studied, parasites are genotypically indistinguishable.