Therapeutic Potential of Bama Pig Adipose-Derived Mesenchymal Stem Cells for the Treatment of Carbon Tetrachloride-Induced Liver Fibrosis

• Published in: Experimental and clinical transplantation Vol. 18; no. 7; pp. 823 - 831
• Main Authors: Wu, Xinran, Zhang, Shuang, Lai, Junhui, Lu, Huidi, Sun, Yuchen, Guan, Weijun
• Format: Journal Article
• Language: English
• Published: Başkent Üniversitesi 01-12-2020
• Subjects: Tıp; Ankara; Türkiye; Animal model; Hepatic differentiation; Carbon tetrachloride-treated mice
• ISSN: 1304-0855; 2146-8427
• DOI: 10.6002/ect.2020.0108

Objectives: Liver fibrosis is inevitable in the healing
process of liver injury. Liver fibrosis will develop
into liver cirrhosis unless the damaging factors are
removed. This study investigated the potential therapy
of Bama pig adipose-derived mesenchymal stem
cells in a carbon tetrachloride-induced liver fibrosis
Institute of Cancer Research strain mice model.
Materials and Methods: Adipose-derived mesenchymal
stem cells were injected intravenously into the tails of
mice of the Institute of Cancer Research strain that had
been treated with carbon tetrachloride for 4 weeks.
Survival rate, migration, and proliferation of adiposederived
mesenchymal stem cells in the liver were
observed by histochemistry, fluorescent labeling, and
serological detection.
Results: At 1, 2, and 3 weeks after adipose-derived
mesenchymal stem cell injection, liver fibrosis was
significantly ameliorated. The injected adiposederived
mesenchymal stem cells had hepatic
differentiation potential in vivo, and the survival rate
of adipose-derived mesenchymal stem cells declined
over time.
Conclusions: The findings in this study confirmed that
adipose-derived mesenchymal stem cells derived from
the Bama pig can be used in the treatment of liver
fibrosis, and the grafted adipose-derived mesenchy -
mal stem cells can migrate, survive, and differentiate
into hepatic cells in vivo.