Association of tumor necrosis factor-α gene polymorphism with osteoarticular tuberculosis prognosis in a Hebei population

• Published in: Genetics and molecular research Vol. 15; no. 4
• Main Authors: Lv, Y J, Liu, S J, Hu, W N, Zhang, G P, Ren, Q Y, Zheng, L D, Zhang, Y C, Li, R Q, Zhang, Z K
• Format: Journal Article
• Language: English
• Published: Brazil 24-10-2016
• Subjects: Adult; Alleles; Female; Genetic Association Studies; Genetic Predisposition to Disease; Genotype; Haplotypes; Humans; Male; Middle Aged; Polymorphism, Single Nucleotide; Prognosis; Risk Factors; Tuberculosis, Osteoarticular - genetics; Tuberculosis, Osteoarticular - pathology; Tumor Necrosis Factor-alpha - genetics
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/gmr15048937

This study investigated the association of tumor necrosis factor-α (TNF-α)-308, -238, and -863 polymorphisms with osteoarticular tuberculosis (OA-TB) prognosis in a Hebei population. Genomic DNA was extracted from venous blood samples of 120 OA-TB patients and 100 healthy volunteers. TNF-α-308, -238, and -863 were analyzed by PCR-restriction fragment length polymorphism; genotype and allele frequencies were calculated. Serum TNF-α level was significantly higher in OA-TB patients (283.16 ± 51.68 ng/L) than in control (122.54 ± 54.65 ng/L; P < 0.05). Higher frequency of TNF-α-308 GG genotype in healthy volunteers (91.0%) than in OA-TB patients (79.2%) indicated that it was a protective factor against OA-TB (OR = 0.405, 95%CI = 0.147-0.657, P = 0.007). Higher frequencies of TNF-α-308 GA genotype and TNF-α-308 allele (A) in OA-TB patients (20.8 and 10.4%, respectively) than in healthy volunteers (8.0 and 5.0%, respectively) indicated an association with increased risk of OA-TB (OR = 3.112, 95%CI = 1.520-6.343, P = 0.003; OR = 3.109, 95%CI = 1.676-6.538, P = 0.006; respectively). Haplotype association analysis of TNF-α polymorphisms (-308/-238/-863) showed a higher frequency of TNF-α AGA in OA-TB patients (12.1%) than in healthy volunteers (3.5%), indicating that it was a risk factor for OA-TB (OR = 4.201, 95%CI = 1.80-9.91, P = 0.010). TNF-α-308 G/A and TNF-α AGA (-308/-238/-863) were associated with a predisposition to OA-TB, which could aid clinical detection, prevention, and prognosis of OA-TB.