Diazirine‐functionalized Nanostructured Platform for Enzymes Photografting and Electrochemical Biosensing

• Published in: Electroanalysis (New York, N.Y.) Vol. 31; no. 8; pp. 1543 - 1551
• Main Authors: Stoytcheva, Margarita, Zlatev, Roumen, Velkova, Zdravka, Gochev, Velizar, Ayala, Alan, Montero, Gisela, Valdez, Benjamín
• Format: Journal Article
• Language: English
• Published: 01-08-2019
• Subjects: Diazirine; Differential Alternative Pulses Voltammetry; Dopamine biosensor; Tyrosinase
• ISSN: 1040-0397; 1521-4109
• DOI: 10.1002/elan.201900086

A simple technique for the construction of a versatile diazirine‐functionalized nanostructured platform for enzymes photografting and electrochemical biosensing was proposed in this work. The feasibility of the approach was proved by photo crosslinking of an enzyme, tyrosinase, to diazirine‐activated aminated carbon nanotubes coated glassy carbon electrode. The analytical performances of the realized biosensor were evaluated employing catechol as analyte. Then the sensor based on the diazirine‐functionalized nanostructured platform with photografted tyrosinase was applied together with the high resolution technique Differential Alternative Pulses Voltammetry for dopamine determination in the linear concentration range of 5–25 μmol L−1 in the presence of interfering agents as uric acid up to its 100‐fold excess.