DNA-dependent RNA polymerase II of plant origin transcribes viroid RNa into full-length copies

DNA-dependent RNA polymerase II of plant origin transcribes viroid RNa into full-length copies

DNA-dependent RNA polymerase II purified from healthy plant tissue is capable of synthesizing linear (-)-viroid RNA copies of full length from (+)-viroid RNA templates in vitro. Together with the specific alpha-amanitin sensitivity of viroid replication observed in vivo, these findings suggest that...

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Bibliographic Details
Published in:Nature (London) Vol. 291; no. 5813; pp. 297 - 301
Main Authors: Rackwitz, H.R, Rohde, W, Sanger, H.L
Format: Journal Article
Language:English
Published: England 28-05-1981
Subjects:
Amanitins - pharmacology
DNA-Directed RNA Polymerases - metabolism
Kinetics
Nucleic Acid Hybridization
plant health
plant viruses
Plants - genetics
Polynucleotides
RNA Polymerase II - metabolism
RNA, Viral - genetics
Templates, Genetic
Transcription, Genetic - drug effects
Viroids - genetics
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Summary:DNA-dependent RNA polymerase II purified from healthy plant tissue is capable of synthesizing linear (-)-viroid RNA copies of full length from (+)-viroid RNA templates in vitro. Together with the specific alpha-amanitin sensitivity of viroid replication observed in vivo, these findings suggest that viroids replicate by an entirely novel mechanism in which infecting viroid RNA molecules are copied by the host enzyme which is normally responsible for the synthesis of nuclear precursors to messenger RNA.
ISSN:0028-0836
1476-4687
DOI:10.1038/291297a0