Clinical significance of novel identified high‐frequency tumor‐specific peptides associated signature in predicting disease status of gastric cancer patients

• Published in: BioFactors (Oxford) Vol. 47; no. 6; pp. 1042 - 1052
• Main Authors: Li, Bin, Geng, Huizhen, Li, Zibo, Peng, Bing, Wang, Jinfeng, Yin, Xiaolei, Li, Ning, Shi, Jianfei, Zhao, Man, Li, Cuizhen, Yin, Fei
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01-11-2021
• Subjects: Adult; Aged; Aged, 80 and over; Biomarkers, Tumor - metabolism; Disease Progression; Female; gastric cancer; Humans; Male; Middle Aged; Peptides - metabolism; Retrospective Studies; serological test; spectrometry; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; tumor differentiation; tumor‐specific target peptides; serological test; spectrometry; gastric cancer; tumor-specific target peptides; tumor differentiation
• ISSN: 0951-6433; 1872-8081
• DOI: 10.1002/biof.1778

The effectively early detection and determination of disease progression of gastric cancer (GC) are still required. An emerging demand for identifying the novel targets adherent to cancer cells has been still challenged since those valuable profilings not only could act as for early gastric tumor discovery but also being potential therapeutic views. We have retrospectively analyzed GC biopsies to identify those specific target peptides in association with disease progression. We have detected the polypeptide by liquid mass technology initiated BIO‐HIGH innovational assay technology for tumor‐specific target peptide identification. We have validated the accessibility and feasibility of multiple target cytotoxic T‐lymphocyte for the assessment of potential molecular markers by equally comparing the frequencies of tumor peptides' loci identified in 138 GC patients. The aim was to separate peripheral blood lymphocytes by density gradient centrifugation and use specific target peptides in in vitro culture of lymphocytes. The Cell Counting Kit‐8 assay was set up to prove the lymphocytes' proliferation stimulated by identified peptides. Both of GC‐specific peptide and shared peptide were detected in the peripheral blood, and the frequencies and quantities were correlated with disease status and cancer differentiation, in which BHGa1510 (78%), BHGa1310 (66%), BHGa0910 (57%), BHGa0310 (54%), BHGa0210 (40%), BHGa0810 (35%), BHGa0110 (33%), and BHGa1410 (30%) were apparently scoped out as high‐frequency (HF) peptides could be potentially specific tumor markers. Moreover, BHGa1410 was significantly associated with cancer progression, and BHGa0910 and BHGa0210 were significantly associated with TNM stage. The IHC data have shown that both the HF BHGa1510 and HF BHGa1310 were expressions by 100% in contrast with paracancerous tissues of 40% (p < 0.05) and 33%, respectively (p < 0.05). Those specific peptide pools could be valued in assessment of advanced tumor and differential status in GC patients.