Raman spectroscopic study of plasma-treated salmon DNA

• Published in: Applied physics letters Vol. 102; no. 2
• Main Authors: Joon Lee, Geon, Kwon, Young-Wan, Hee Kim, Yong, Ha Choi, Eun
• Format: Journal Article
• Language: English
• Published: United States 14-01-2013
• Subjects: ABSORPTION; CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; Deoxyribonucleic acid; DEOXYRIBOSE; DICHROISM; DNA; EMISSION SPECTRA; INTERACTIONS; IONS; MAGNETIC CIRCULAR DICHROISM; MODIFICATIONS; MOLECULAR STRUCTURE; Optical properties; PHOSPHATES; PLASMA; Plasma (physics); RAMAN SPECTRA; SALMON; Salmonidae; SOLIDS; THIN FILMS; ULTRAVIOLET RADIATION; Vibration mode
• ISSN: 0003-6951; 1077-3118
• DOI: 10.1063/1.4776673

In this research, we studied the effect of plasma treatment on the optical/structural properties of the deoxyribonucleic acid (DNA) extracted from salmon sperm. DNA-cetyltrimethylammonium (CTMA) films were obtained by complexation of DNA with CTMA. Circular dichroism (CD) and Raman spectra indicated that DNA retained its double helical structure in the solid film. The Raman spectra exhibited several vibration modes corresponding to the nuclear bases and the deoxyribose-phosphate backbones of the DNA, as well as the alkylchains of CTMA. Dielectric-barrier-discharge (DBD) plasma treatment induced structural modification and damage to the DNA, as observed by changes in the ultraviolet-visible absorption, CD, and Raman spectra. The optical emission spectra of the DBD plasma confirmed that DNA modification was induced by plasma ions such as reactive oxygen species and reactive nitrogen species.