Plasmid maintenance and recombinant cell fitness explored in bacterial colonies

During growth of recombinant bacteria, irregular plasmid partitioning generates non-productive, plasmid-free cells whose proportion usually increases in the culture. For Escherichia coli producing engineered β-galactosidases, we have shown a coincidence between plasmid stability and the extension of...

Full description

Saved in:

Bibliographic Details
Published in:	Biotechnology letters Vol. 23; no. 10; pp. 831 - 838
Main Authors:	CAZORLA, Daniel, FERRER-MIRALLES, Neus, FELIU, Jordi X, CARBONELL, Xavier, VILLAVERDE, Antonio
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer 01-05-2001 Springer Nature B.V
Subjects:	Bacteria Biological and medical sciences Biotechnology E coli Escherichia coli Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics lacZ gene Methods. Procedures. Technologies Microbiology Miscellaneous Synthetic digonucleotides and genes. Sequencing Recombinant microorganism Microorganism growth Stability Enzyme Escherichia coli Plasmid Glycosidases Microorganism culture Bacteria Hydrolases O-Glycosidases β-Galactosidase Enterobacteriaceae Fitness
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Be the first to leave a comment!