Testing the 3′-untranslated RNA regions of beet necrotic yellow vein virus and beet yellows virus as inducers of posttranscriptional gene silencing

• Published in: Doklady. Biochemistry and biophysics Vol. 439; no. 1; pp. 195 - 198
• Main Authors: Vinogradova, S. V., Kamionskaya, A. M., Rakitin, A. L., Agranovsky, A. A., Ravin, N. V., Atabekov, J. G., Skryabin, K. G.
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 01-08-2011; Springer Nature B.V
• Subjects: 3' Untranslated Regions - genetics; Agrobacterium tumefaciens - genetics; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Biophysics and Molecular Biology; Closterovirus - genetics; Genetic Engineering - methods; Life Sciences; Nicotiana - genetics; Plant diseases; Plant resistance; Plants, Genetically Modified; RNA Interference; RNA, Viral - genetics; Transformation, Genetic; Viruses; Benthamiana Plant; Beet Necrotic Yellow Vein Virus; DOKLADY Biochemistry; Posttranscriptional Gene Silence; Target Construct
• ISSN: 1607-6729; 1608-3091
• DOI: 10.1134/S1607672911040144

Vinogradova et al studied the effectiveness of using the 3' untranslated region of the beet necrotic yellow vein virus and beet yellows virus genomic RNA for the induction of RNA silencing. To analyze the suppression of the expression level of viral genome fragments, they developed a procedure for transient and stable expression in plants of the reporter gene GFP, which was fused to BYV or BNYVV specific sequence, and viral double stranded RNAs that potentially induce silencing.