Quantitative determination of diethylstilboestrol in bovine urine with isotope dilution mass spectrometry

A method has been described for the quantification of diethylstilboestrol (DES) in bovine urine. The determining step is based on isotope dilution mass spectrometry (IDMS). The chemical work-up consisted of enzymatic deconjugation, Sep-Pak clean-up and high-performance liquid chromatography (HPLC)....

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Bibliographic Details
Published in:	Biomedical & environmental mass spectrometry Vol. 13; no. 5; p. 245
Main Authors:	Jansen, E H, Freudenthal, J, van Rossum, H J, Litjens, J L, Stephany, R W
Format:	Journal Article
Language:	English
Published:	England 01-05-1986
Subjects:	Animals Cattle Chromatography, High Pressure Liquid Diethylstilbestrol - analogs & derivatives Diethylstilbestrol - urine Hydrolysis Indicator Dilution Techniques Mass Spectrometry
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Summary:	A method has been described for the quantification of diethylstilboestrol (DES) in bovine urine. The determining step is based on isotope dilution mass spectrometry (IDMS). The chemical work-up consisted of enzymatic deconjugation, Sep-Pak clean-up and high-performance liquid chromatography (HPLC). The labelled compound synthesized for IDMS is hexadeutero DES (DES-2H6). The labelled compound is not only used to quantify DES, but also to check the recovery of the chemical work-up procedure, in order to avoid false negative results. At the 1-5 micrograms l-1 level of urinary DES, the average recovery of 2 micrograms l-1 DES-2H6 is 70 +/- 20% (s.d.).
ISSN:	0887-6134
DOI:	10.1002/bms.1200130507