Biosynthesis of prostaglandins by human spermatozoa in vitro and their role in acrosome reaction and fertilization

Biosynthesis of prostaglandins by human spermatozoa in vitro and their role in acrosome reaction and fertilization

Five homogenates of human sperm cells were separately incubated with [14C]arachidonic acid in the presence of reduced glutathione, L-tryptophan, and haematin as cofactors. The cyclooxygenase products of arachidonic acid metabolism were extracted, separated, and measured for their radioactivity. The...

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Bibliographic Details
Published in:Molecular reproduction and development Vol. 33; no. 3; p. 303
Main Authors: Roy, A C, Ratnam, S S
Format: Journal Article
Language:English
Published: United States 01-11-1992
Subjects:
Acrosome - metabolism
Arachidonic Acid - metabolism
Calcium - metabolism
Fertilization - physiology
Humans
In Vitro Techniques
Male
Prostaglandin-Endoperoxide Synthases - metabolism
Prostaglandins - biosynthesis
Spermatozoa - metabolism
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Summary:Five homogenates of human sperm cells were separately incubated with [14C]arachidonic acid in the presence of reduced glutathione, L-tryptophan, and haematin as cofactors. The cyclooxygenase products of arachidonic acid metabolism were extracted, separated, and measured for their radioactivity. The rate of formation of prostaglandin (PG)D2, PGE2, PGF2 alpha, 6-keto PGF1 alpha, and thromboxane (TX)B2 were 18.0 +/- 1.11, 10.9 +/- 0.68, 5.8 +/- 0.21, 3.9 +/- 0.13 and 6.6 +/- 0.52 pmol/10(6) cells/min, respectively. These results are discussed in relation to the hypothesis that cyclooxygenase metabolites of certain polyunsaturated fatty acids play an important part in the sperm acrosome reaction and fertilization.
ISSN:1040-452X
DOI:10.1002/mrd.1080330311