Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)

We report a one-pot, closed-vessel enzymatic assay that eliminates carryover contamination while preserving robust DNA amplification in loop-mediated isothermal amplification (LAMP), providing reliable and rapid detection of target DNA in contaminated samples.

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Bibliographic Details
Published in:	Chemical communications (Cambridge, England) Vol. 50; no. 28; p. 3747
Main Authors:	Hsieh, Kuangwen, Mage, Peter L, Csordas, Andrew T, Eisenstein, Michael, Soh, H Tom
Format:	Journal Article
Language:	English
Published:	England 11-04-2014
Subjects:	DNA, Bacterial - analysis DNA, Bacterial - chemistry Nucleic Acid Amplification Techniques - methods Salmonella typhimurium - genetics Uracil-DNA Glycosidase - chemistry
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Description
Summary:	We report a one-pot, closed-vessel enzymatic assay that eliminates carryover contamination while preserving robust DNA amplification in loop-mediated isothermal amplification (LAMP), providing reliable and rapid detection of target DNA in contaminated samples.
ISSN:	1364-548X
DOI:	10.1039/c4cc00540f