Accumulation of WCS120 protein in wheat cultivars grown at 9°C or 17°C in relation to their winter survival

• Published in: Plant breeding Vol. 129; no. 6; pp. 611 - 616
• Main Authors: VITAMVAS, P, KOSOVA, K, PRASILOVA, P, PRASIL, I. T
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-12-2010; Blackwell
• Subjects: Adaptation to environment and cultivation conditions; Agronomy. Soil science and plant productions; Biological and medical sciences; frost tolerance; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; Triticum aestivum; Varietal selection. Specialized plant breeding, plant breeding aims; WCS120 protein; winter survival; Monocotyledones; Overwintering; Survival; Cereal crop; Protein; winter survival; Gramineae; Angiospermae; Genetic improvement; Spermatophyta; WCS120 protein; Triticum aestivum; frost tolerance; Cultivar; Frost resistance
• ISSN: 0179-9541; 1439-0523
• DOI: 10.1111/j.1439-0523.2010.01783.x

With 4 figures and 1 table ABSTRACT: We studied how temperature treatments, at 9°C or 17°C during plant growth under controlled conditions, affected the accumulation of the cold‐regulated dehydrin WCS120 in 21 wheat cultivars which had different winter survival rates. Cultivar survival was established using the provocation pot method over three winters. The levels of WCS120 were determined by immunoblot analyses. There was a good correlation between winter survival and the accumulation of WCS120 in the different cultivars of wheat grown under either 9°C or 17°C. Those plants grown at 9°C accumulated higher amounts of WCS120 than did those grown at 17°C. However, all cultivars tested, including the spring variety, accumulated the WCS120 protein at 17°C. These results indicate that the wheat cultivars with higher levels of frost tolerance can be differentiated from the less‐tolerant ones by the evaluation of WCS120 protein content in the plants, when grown at temperatures higher than those that are normally used in cold‐acclimation treatments.