High Prevalence of Colistin-Resistant Encoding Genes Carriage among Patients and Healthy Residents in Vietnam

• Published in: Medicina (Kaunas, Lithuania) Vol. 60; no. 7; p. 1025
• Main Authors: Le, Viet Ha, Khong, Thi Diep, Phan, Ngoc Quang, Tran, Thi Hoa, Vu, Hong Ngoc, Van Quyen, Dong, Hoang, Van Thuan, Nguyen, Nam Thang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21-06-2024
• Subjects: Adult; Aged; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Antibiotics; Antimicrobial agents; Bacteria; Colistin - pharmacology; Colistin - therapeutic use; colistin-resistant encoding genes; Drug resistance; Drug Resistance, Bacterial - genetics; Feces - microbiology; Female; Genes; Genetic testing; Health care; Health facilities; Humans; Infections; Infectious diseases; Male; mcr; Microbial Sensitivity Tests; Middle Aged; Plasmids; Prevalence; Public health; Vietnam; Vietnam - epidemiology; Vietnam; United States > US; Germany; colistin-resistant encoding genes; mcr; Vietnam
• ISSN: 1648-9144; 1010-660X; 1648-9144
• DOI: 10.3390/medicina60071025

: We aimed to investigate the carriage of colistin-resistant genes among both patients with a history of antibiotic exposure and apparently healthy adults with no recent healthcare contact.
: Stool swabs were collected from healthy people, and specimens were collected at the infection foci from the patients. Eleven primer/probe sets were used to perform the Multiplex Real-Time PCR assay with the QuantiNova Multiplex Probe PCR kit for screening the carriage of colistin-resistant genes (
-1 to
-10) and
gene as internal control.
: In total, 86 patients and 96 healthy residents were included. Twenty two patients (25.9%) were positive with at least one colistin-resistance encoding gene. The
-1 gene was the most frequent (16.5%), followed by
-9,
-6, and
-4 genes, where the prevalence was 11.8%, 10.6%, and 9.4%, respectively. No patient was positive with
-3,
-7, and
-8 genes. Eight patients (9.4%) were positive with multiple colistin-encoding genes. Twenty-three healthy people (24.0%) were positive with at least one colistin-resistance encoding gene, and the
-10 gene was the most frequent (27.0%), followed by the
-1,
-8, and
-9 genes, where the prevalence was 24.3%, 21.6%, and 13.5%, respectively. No person was positive with the
-2 and
-5 genes.
: Our findings underscore the urgent need for enhanced surveillance, infection control measures, and stewardship interventions to mitigate the spread of colistin resistance in Vietnam.