The impact of FSH stimulation and age on the ovarian and uterine traits and histomorphometry of prepubertal gilts

• Published in: Domestic animal endocrinology Vol. 83; p. 106786
• Main Authors: Alfradique, V.A.P., Netto, D.L. Souza, Alves, S.V.P., Machado, A.F., Novaes, C.M., Penitente-Filho, J.M., Machado-Neves, M., Lopes, M.S., Guimarães, S.E.F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-04-2023
• Subjects: Animals; B-mode ultrasonography; Biometry; Estradiol; Estradiol - pharmacology; Female; Follicle Stimulating Hormone; Follicle Stimulating Hormone, Human; Gonadotropin treatment; Luteinizing Hormone; Morphology; Ovarian Follicle - physiology; Ovary; Pig; Sus scrofa; Swine; Uterus; GEH; HT; Estradiol; E2; GT; Biometry; LEH; Pig; ET; B-mode ultrasonography; Morphology; Gonadotropin treatment; FSH; GSI; LH; GD
• ISSN: 0739-7240; 1879-0054
• DOI: 10.1016/j.domaniend.2023.106786

•FSH treatment alters endometrial histomorphometry in prepubertal gilts.•Changes in uterine morphology do not occur from 140 to 160 d of age in prepubertal gilts.•Ovarian preantral follicles are not responsive to FSH treatment in prepubertal gilts.
This study investigated the effect of age and follicle stimulating hormone (FSH) treatment on the estradiol (E2) plasma concentration, ovarian follicle development, endometrial histomorphometry, and ultrasonographic parameters of the ovaries and uterus in prepubertal gilts. Thirty-five prepubertal gilts were grouped according to age (140 or 160 d), and within each age, gilts were allotted to receive 100 mg of FSH (treated; G140 + FSH [n = 10] and G160 + FSH [n = 7]) or saline solution (control; G140 + control [n = 10] and G160 + control [n = 8]). The total dose of FSH was divided into 6 similar doses administered every 8 h (days 0–2). Before and after FSH treatment, blood sample was collected, and transabdominal scanning of the ovaries and uterus was performed. Twenty-four hours after the last FSH injection, the gilts were slaughtered and their ovaries and uterus were processed for histological and histomorphometric analysis. The histomorphometric parameters of the uterus differed (P < 0.05) between prepubertal gilts at 160 d and 140 d of age. Moreover, changes (P < 0.05) in uterine and ovarian ultrasound images occurred between 140 and 160 d of age. Age and FSH treatment did not affect (P > 0.05) E2 plasma concentrations. Follicle stimulating hormone treatment did not affect (P > 0.05) the early stage of folliculogenesis in the prepubertal gilts; however, the number of early atretic follicles decreased (P < 0.05) after the FSH treatment. Follicle stimulating hormone administration increased (P < 0.05) the number of medium follicles and decreased (P < 0.05) the number of small follicles in 140 and 160 d old gilts. In the endometrium, luminal/glandular epithelium height and glandular diameter increased (P < 0.05) after FSH treatment. Thus, injections of 100 mg of FSH stimulate the endometrium epithelium and induce follicular growth to a medium follicle size without affecting the preantral stages in prepubertal gilts; also, the uterine macroscopic morphometry does not change from 140 to 160 d of age.