Computational Guided Method Applied to LSPR‐Based Biosensor for Specific Detection of the Four‐Serotypes of Dengue Virus in Seropositive Patients

• Published in: Particle & particle systems characterization Vol. 39; no. 3
• Main Authors: Machado, Gabriel L., Teixeira, Felipe M. F., Ferreira, Gabriel S. C., Versiani, Alice F., Andrade, Lidia M., Ladeira, Luiz O., da Fonseca, Flávio G., Ramirez, Jhonattan C.
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley Subscription Services, Inc 01-03-2022
• Subjects: Biosensors; Chaos theory; Dengue fever; Encephalitis; flavivirus; Liapunov exponents; LSPR biosensors; Nanoparticles; Nanorods; numerical modeling; plasmonic; Surface plasmon resonance; Viruses
• ISSN: 0934-0866; 1521-4117
• DOI: 10.1002/ppsc.202100157

In this paper, a localized surface plasmon resonance (LSPR)‐based biosensor, computationally guided to detect selectively the four Dengue virus (DENVx) serotypes in seropositive patients, is presented. The behavior of gold nanoparticles in the shape of nanorods is theoretically and numerically studied as a function of induced structural variations, which are experimentally evidenced due to the bio‐interaction between the target analytes and its surface during the detection process. Additionally, with the implementation of the largest Lyapunov exponent, it is possible to calculate the notion of predictability for the experimental results, observing chaotic systems with a very low probability of repetition. Due to the above, when analyzing the recurrence map associated with the obtained resonance curve generated by the LSPR system, the genetic similarity of DENV3/DENV2 and DENV4/DENV1 is evidenced. Finally, the biosensors are validated by analyzing samples of seronegative patients for DENVx and seropositive ones for other Flaviviruses such as Zika virus, Yellow Fever virus, and Saint Louis Encephalitis virus.
In this paper, an original computational approach to selectively detect each of the four Dengue virus serotypes in seropositive patients in the presence of other flaviviruses is presented. Similarly, and due to the chaotic nature of the experimental system, it is demonstrated the genetic similarity between DENV3/DENV2, and DENV4/DENV1, implementing recurrence map analysis in the measured resonance curve.